Brassinosteroid biosynthesis and signalling in petunia hybrida

Seed germination performance is a major determinant of crop yield. Deterioration of seed quality with age is associated with accumulation of DNA damage . [6] In dry, aging rye seeds, DNA damages occur with loss of viability of embryos. [7] Dry seeds of Vicia faba accumulate DNA damage with time in storage, and undergo DNA repair upon germination. [8] In Arabidopsis , a DNA ligase is employed in repair of DNA single- and double-strand breaks during seed germination and this ligase is an important determinant of seed longevity. [9] In eukaryotes , the cellular repair response to DNA damage is orchestrated, in part, by the DNA damage checkpoint kinase ATM . ATM has a major role in controlling germination of aged seeds by integrating progression through germination with the repair response to DNA damages accumulated during the dry quiescent state. [10]

For MTT assays, cells (2,000 ~ 5,000 cells/well) were subcultured into 96-well plates according to their growth properties. Cell proliferation was assayed at 72 hr after treatment of CHIR-99021 by adding 20 μl of 5 mg/ml 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution per 100 μl of growth medium. After incubating for 3-4 h at 37°C, the media were removed and 150 µl/well of MTT solvent (either absolute DMSO or isopropanol containing 4 mM HCl and % Nonidet-40) was added to dissolve the formazan. The absorbance of each well was measured by ELx808 (BioTek, Winooski, VT) or Wallac Victor2 (Perkin-Elmer Life Sciences, Boston, MA) Microplate Reader. Viable cells are presented as percent of control, vehicle-treated cells. 

Open Access journals are the major source of knowledge for young and aspiring generations who are keen in pursuing a career in sciences. This system provides easy access to networks of scientific journals . Authors that contribute their scholarly works to Open Access journals gain remarkable reputation as the research scholarly explore these works extensively. This process assures considerable impact factor for the journal and reputation to the authors that add value to their Academic Performance Index (API) Score. Because of the free access open access journals impact factors are improving.

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Brassinosteroid biosynthesis and signalling in petunia hybrida

brassinosteroid biosynthesis and signalling in petunia hybrida

This site is part of The Superman Diet © 2015 All Rights Reserved. Privacy | Terms | Disclaimer

All content posted on this site is commentary or opinion and is protected under Free Speech. The Superman Diet is not responsible for content written by contributing authors. The information on this site is provided for educational and entertainment purposes only. It is not intended as a substitute for professional advice of any kind. The Superman Diet assumes no responsibility for the use or misuse of this material. Your use of this website indicates your agreement to these terms and those published here. All trademarks, registered trademarks and servicemarks mentioned on this site are the property of their respective owners.

The statements made on this website have not been evaluated by the FDA (. Food & Drug Administration).

The information published on this site is not intended to diagnose, treat, cure, or prevent any disease.

The information provided by this website and/or this company is not a substitute for a face-to-face consultation with your physician, and should not be construed as individual medical advice.

The entire contents of this website are based upon the opinions of the author, unless otherwise noted. The information on this website is not intended to replace a one-on-one relationship with a qualified health care professional and is not intended as medical advice. It is intended as a sharing of knowledge and information from the research and experience of the author and his community. The author encourages you to make your own health care decisions based upon your research and in partnership with a qualified health care professional.

will attempt to keep all objectionable messages off this site; however, it is impossible to review all messages immediately. All messages expressed on this site, including comments posted to Blog entries, represent the views of the author exclusively and will not be held responsible for the content of any message.

By agreeing to these rules, you warrant that you will not post any messages that are obscene, vulgar, sexually-oriented, hateful, threatening, or otherwise violative of any laws.

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