Steroidale saponine

Pre-exposure to 25 µg SAP ml −1 did not affect ( P  > 0·05) the growth curves of any of the four non-cellulolytic bacterial species examined ( Fig. 1 ). Including 250 µg SAP ml −1 in the medium, however, reduced the growth of Strep. bovis between 2 and 8 h ( P  < 0·01) and P. bryantii at 4, 5, 6, 8 and 10 h ( P  < 0·05), relative to growth without SAP. Including 250 µg ml −1 SAP did not affect ( P  > 0·05) the growth of Rb. amylophilus up to 10 h. However, a negative effect ( P  < 0·05) of SAP on Rb. amylophilus was observed at 14 and 24 h and on P. bryantii at 24 h. In contrast, SAP enhanced ( P  < 0·05) the growth of S. ruminantium between 8 and 10 h, and cultures containing SAP maintained a higher ( P  < 0·05) optical density at 14 and 24 h.

Steroidale saponine

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